Could polymerase chain reaction be an alternative diagnostic method for dermatophytes?
نویسندگان
چکیده
Dermatophytosis are cutaneous mycoses caused by Microsporum spp., Trichophyton spp. and Epidermophyton dermatophytes. resembles other skin diseases due to its various clinical manifestations such as multifocal alopecia, circular lesions, scaling, crusting, papular pustular follicular obstruction, erythema, hyperpigmentation, miliary dermatitis dystrophic nail growth, diagnosis is based on the use of many different methods. Generally used methods; appearance, microscopic examination, examination with Wood's lamp mycological culture but these methods have some disadvantages. Microscopic requiring expertise, fast cost-effective method, in cases, microbiologists encounter specimens that microscopically negative give positive results culture. Examination can only be for M. canis specificity low. On hand cultures require a long time (3-6 weeks) definitive results, their sensitivity may decrease common contaminant growth. Considering reasons, new polymerase chain reaction (PCR) been developed dermatophyte agents. Compared molecular methods, PCR method easy, applicable identification species do not show typical morphological features. Although PCR-based diagnostic widely humans dermatophytosis, usefulness dogs cats has also confirmed. As result, dermatophytosis; it emphasized dermatophytosis ease obtaining samples, providing faster compared culture, should diseases. In this study, was aimed demonstrate effectiveness applicability practice, well which frequently dermatophytosis.
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ژورنال
عنوان ژورنال: Journal of istanbul veterinary sciences
سال: 2022
ISSN: ['2602-3490']
DOI: https://doi.org/10.30704/http-www-jivs-net.1184780